Utility and diagnostic performance of Mycobacterium tuberculosis complex by two immunochromatographic assays as compared with the molecular Genotype assay in Nigeria

Benjamin Thumamo Pokam; Anne E Asuquo; Khye Seng Goh; Lydia N Abia-Bassey; Nalin Rastogi

doi:10.1016/j.ijmyco.2012.12.002

Utility and diagnostic performance of Mycobacterium tuberculosis complex by two immunochromatographic assays as compared with the molecular Genotype assay in Nigeria

Int J Mycobacteriol. 2013 Mar;2(1):34-7. doi: 10.1016/j.ijmyco.2012.12.002. Epub 2013 Jan 25.

Authors

Benjamin Thumamo Pokam¹, Anne E Asuquo², Khye Seng Goh³, Lydia N Abia-Bassey², Nalin Rastogi³

Affiliations

¹ Department of Medical Laboratory Science, Faculty of Health Sciences, University of Buea, Cameroon. Electronic address: thumamo@yahoo.fr.
² Department of Medical Laboratory Science, College of Medical Sciences, University of Calabar, Nigeria.
³ WHO Supranational TB Reference Laboratory, TB & Mycobacteria Unit, Institut Pasteur de la Guadeloupe, 97183 Abymes, Guadeloupe, France.

PMID: 26785786
DOI: 10.1016/j.ijmyco.2012.12.002

Abstract

Among the disadvantages of smear microscopy for detection of tuberculosis cases is its inability to differentiate between Mycobacterium tuberculosis (MTB) and non-tuberculous mycobacteria (NTM). This study evaluated two, new immunochromatographic assays - Capilia TB-Neo and SD Bioline - on unheated and heated cultures at 80°C for 30min respectively for their ability to discriminate between MTB complex and NTM as compared with the molecular Genotype assay. Mycobacteria used in the study were obtained from smear-positive specimens collected from patients at four major hospitals in Cross River State, Nigeria. Capilia TB-Neo and SD Bioline showed sensitivities of 98.8% and 93.8% respectively and 100% specificity for both assays. Heating the isolates did not significantly impact the test performance. Both tests are recommended for use in rapid differentiation of strains isolated in Nigeria.

Keywords: Capilia TB-Neo; Heated strains; Mycobacterium tuberculosis complex; Non-tuberculous mycobacteria; SD Bioline.