Fish represent the largest and most diverse group of vertebrates. Their evolutionary position relative to other vertebrates and their ability to adapt to a wide variety of environments make them ideal for studying both organismic and molecular evolution. A number of other characteristics make them excellent experimental models for studies in embryology, neurobiology, endocrinology, environmental biology, and other areas. In fact, they have played a critical role in the development of several of these disciplines. Research techniques that enable scientists to make isogenic lines in a single generation, create and maintain mutants, culture cells, and transfer cloned genes into embryos signal an increasing role for fish as experimental models.