Abstract
In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with higher quantities found in the culture supernatants. A hemolytic assay showed that the recombinant strain secreted 250 ng active LLO/mg total protein. This mInlA/LLO-producing strain of L. lactis may be used as an alternative tool in DNA vaccination against a number of infectious diseases or in cancer therapy.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / immunology*
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Bacterial Toxins / genetics*
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Bacterial Toxins / immunology*
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Bacterial Vaccines
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Cell Membrane / metabolism
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Gene Expression
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Heat-Shock Proteins / genetics*
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Heat-Shock Proteins / immunology*
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Hemolysin Proteins / genetics*
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Hemolysin Proteins / immunology*
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Hemolysis
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Lactococcus lactis / genetics*
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Lactococcus lactis / metabolism
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Listeria monocytogenes / immunology*
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Mutation*
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Recombinant Proteins*
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Vaccination
Substances
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Bacterial Proteins
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Bacterial Toxins
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Bacterial Vaccines
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Heat-Shock Proteins
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Hemolysin Proteins
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Recombinant Proteins
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hlyA protein, Listeria monocytogenes