The present study evaluated the impact of cesium ((133)Cs) at four concentrations (0, 0.001, 0.01, and 0.1 mg L(-1)) on growth, concentrations of chlorophyll and carotenoid pigments, and oxidative stress responses in the charophyte, Nitella pseudoflabellata, over 30 days. Oxidative stress was quantified by measuring anti-oxidant enzyme activities and H2O2 content. When compared with the control, significantly elevated activity levels of the anti-oxidative enzymes ascorbic peroxidase, catalase and guaiacol peroxidase were observed at 0.1 mg L(-1) (all p < 0.05), even though the H2O2 level was not significantly elevated. Carotenoid and chlorophyll a and b pigment levels were significantly reduced (all p < 0.05) at Cs exposures of 0.01 and 0.1 mg L(-1). Photosynthetic efficiency (i.e., Fv/Fm) was significantly reduced (p < 0.05) at Cs concentrations ≥0.001 mg L(-1). Significant reduction (p < 0.05) of plant growth (i.e., shoot length) was also observed after 1 week of exposure at Cs concentrations ≥0.001 mg L(-1). Our results suggested that Cs exposure reduced plant growth and affected plant functioning via activating the defense mechanism against oxidative stress in Nitella.
Keywords: Antioxidant enzymes; Cesium; Nitella pseudoflabellata; Oxidative stress.