Exploiting eco-physiological niche to facilitate the separation of the freshwater cyanobacteria Microcystis sp. and Synechococcus sp

J Microbiol Methods. 2016 Mar:122:13-5. doi: 10.1016/j.mimet.2016.01.003. Epub 2016 Jan 9.

Abstract

In a novel approach to separate the co-occurring freshwater cyanobacteria Microcystis and Synechoccous, published ecological characteristics are used to manipulate temperature and nutrient concentrations to successfully establish a unialgal Microcystis strain. The simple protocol has implications for future cyanobacterial culturing approaches and the establishment of new cyanobacteria strains.

Keywords: Cyanobacteria; Isolation; Microcystis; Purification; Synechococcus.

MeSH terms

  • Bacteriological Techniques / methods*
  • Biomass
  • Culture Media, Conditioned
  • Flow Cytometry / methods
  • Food
  • Fresh Water / microbiology*
  • Hydrobiology
  • Microcystis / cytology
  • Microcystis / isolation & purification*
  • Microcystis / physiology
  • Microscopy, Fluorescence / methods
  • Synechococcus / cytology
  • Synechococcus / isolation & purification*
  • Synechococcus / physiology
  • Temperature
  • Water Microbiology*

Substances

  • Culture Media, Conditioned