Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides

Daniela Verga; Moritz Welter; Andreas Marx

doi:10.1016/j.bmcl.2015.12.082

Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides

Bioorg Med Chem Lett. 2016 Feb 1;26(3):841-844. doi: 10.1016/j.bmcl.2015.12.082. Epub 2015 Dec 24.

Authors

Daniela Verga¹, Moritz Welter¹, Andreas Marx²

Affiliations

¹ Department of Chemistry and Konstanz Research School Chemical Biology University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
² Department of Chemistry and Konstanz Research School Chemical Biology University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany. Electronic address: andreas.marx@uni-konstanz.de.

PMID: 26774580
DOI: 10.1016/j.bmcl.2015.12.082

Abstract

DNA polymerases can efficiently and sequence selectively incorporate oligonucleotide (ODN)-modified nucleotides and the incorporated oligonucleotide strand can be employed as primer in rolling circle amplification (RCA). The effective amplification of the DNA primer by Φ29 DNA polymerase allows the sequence-selective hybridisation of the amplified strand with a G-quadruplex DNA sequence that has horse radish peroxidase-like activity. Based on these findings we develop a system that allows DNA detection with single-base resolution by naked eye.

Keywords: DNA polymerase; Naked-eye detection; Oligonucleotide-modified nucleotide; Rolling circle amplification; Single-base resolution.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biotin / chemistry
DNA / analysis*
DNA, Catalytic / metabolism
DNA-Directed DNA Polymerase / metabolism
Escherichia coli / enzymology
Nucleic Acid Amplification Techniques*
Oligonucleotides / chemistry
Oligonucleotides / metabolism*

Substances

DNA, Catalytic
Oligonucleotides
Biotin
DNA
DNA-Directed DNA Polymerase