Abstract
The in-house Carba-NP and Blue-Carba tests were compared using 30 carbapenemase- and 33 non-producing Enterobacteriaceae. Tests were read by three operators. 100% sensitivity was reported for both tests, but Carba-NP was slightly more specific than Blue-Carba (98.9% vs. 91.7%). We describe potential sources of error during tests' preparation and reading.
Keywords:
Carbapenemases; Carbapenems; Diagnostics; Klebsiella; MBL; MIC; NDM; OXA-48; VIM.
Copyright © 2016 Elsevier B.V. All rights reserved.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / analysis*
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Bacterial Proteins / biosynthesis
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Bacterial Typing Techniques / methods
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Bacteriological Techniques / methods*
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Carbapenems / analysis
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Carbapenems / metabolism
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Enterobacteriaceae / enzymology*
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Enterobacteriaceae / isolation & purification*
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Enterobacteriaceae Infections / diagnosis
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Enterobacteriaceae Infections / microbiology
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Reagent Kits, Diagnostic*
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Sensitivity and Specificity
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Time Factors
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beta-Lactamases / analysis*
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beta-Lactamases / biosynthesis
Substances
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Bacterial Proteins
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Carbapenems
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Reagent Kits, Diagnostic
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beta-Lactamases
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carbapenemase