Comparison of the in-house made Carba-NP and Blue-Carba tests: Considerations for better detection of carbapenemase-producing Enterobacteriaceae

J Microbiol Methods. 2016 Mar:122:33-7. doi: 10.1016/j.mimet.2016.01.002. Epub 2016 Jan 7.

Abstract

The in-house Carba-NP and Blue-Carba tests were compared using 30 carbapenemase- and 33 non-producing Enterobacteriaceae. Tests were read by three operators. 100% sensitivity was reported for both tests, but Carba-NP was slightly more specific than Blue-Carba (98.9% vs. 91.7%). We describe potential sources of error during tests' preparation and reading.

Keywords: Carbapenemases; Carbapenems; Diagnostics; Klebsiella; MBL; MIC; NDM; OXA-48; VIM.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / analysis*
  • Bacterial Proteins / biosynthesis
  • Bacterial Typing Techniques / methods
  • Bacteriological Techniques / methods*
  • Carbapenems / analysis
  • Carbapenems / metabolism
  • Enterobacteriaceae / enzymology*
  • Enterobacteriaceae / isolation & purification*
  • Enterobacteriaceae Infections / diagnosis
  • Enterobacteriaceae Infections / microbiology
  • Reagent Kits, Diagnostic*
  • Sensitivity and Specificity
  • Time Factors
  • beta-Lactamases / analysis*
  • beta-Lactamases / biosynthesis

Substances

  • Bacterial Proteins
  • Carbapenems
  • Reagent Kits, Diagnostic
  • beta-Lactamases
  • carbapenemase