Involvement of rice histone deacetylase HDA705 in seed germination and in response to ABA and abiotic stresses

Jinhui Zhao; Mingzhi Li; Dachuan Gu; Xuncheng Liu; Jianxia Zhang; Kunlin Wu; Xinhua Zhang; Jaime A Teixeira da Silva; Jun Duan

doi:10.1016/j.bbrc.2016.01.016

Involvement of rice histone deacetylase HDA705 in seed germination and in response to ABA and abiotic stresses

Biochem Biophys Res Commun. 2016 Feb 5;470(2):439-444. doi: 10.1016/j.bbrc.2016.01.016. Epub 2016 Jan 7.

Authors

Jinhui Zhao¹, Mingzhi Li¹, Dachuan Gu¹, Xuncheng Liu², Jianxia Zhang², Kunlin Wu², Xinhua Zhang², Jaime A Teixeira da Silva³, Jun Duan⁴

Affiliations

¹ Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China; University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, China.
² Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
³ P. O. Box 7, Miki-cho Post Office, Ikenobe 3011-2, Kagawa-ken, 761-0799, Japan.
⁴ Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China. Electronic address: duanj@scib.ac.cn.

PMID: 26772883
DOI: 10.1016/j.bbrc.2016.01.016

Abstract

Histone acetylation and deacetylation play crucial roles in the modification of chromatin structure and regulation of gene expression in eukaryotes. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) assist to maintain the balance of chromatin acetylation status. Previous studies showed that plant HDACs are key regulators involved in response to development and stresses. In this study, we examined the expression pattern and function of HDA705, a member of the RPD3/HDA1-type HDAC in rice. Overexpression of HDA705 in rice decreased ABA and salt stress resistance during seed germination. Delayed seed germination of HDA705 overexpression lines was associated with down-regulated expression of GA biosynthetic genes and up-regulation of ABA biosynthetic genes. Moreover, overexpression of HDA705 in rice enhanced osmotic stress resistance during the seedling stage. Our findings demonstrate that HDA705 may play a role in regulating seed germination and the response to abiotic stresses in rice.

Keywords: Epigenetic; Germination; HDAC; Oryza sativa L; Stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abscisic Acid / pharmacology
Gene Expression Regulation, Developmental / drug effects
Gene Expression Regulation, Developmental / physiology
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Enzymologic / physiology
Gene Expression Regulation, Plant / drug effects
Gene Expression Regulation, Plant / physiology
Germination / physiology*
Histone Deacetylase 1 / metabolism*
Oryza / drug effects
Oryza / physiology*
Osmotic Pressure / physiology*
Salt Tolerance / drug effects
Salt Tolerance / physiology*
Seedlings / drug effects
Seedlings / physiology*
Stress, Physiological / drug effects
Stress, Physiological / physiology

Substances

Abscisic Acid
Histone Deacetylase 1