Objective: To establish the HPLC fingerprint of Clerodendrum lindleyi in order to provide the basis for its quality standard.
Methods: The chromatographic fingerprint was obtained with Angilent Zorbax C18 (250 mm x 4.6 mm, 5 μm) column and gradiently eluted with acetonitrile-0.1% phosphoric acid solution. The column temperature was maintained at 35 degrees C. The flow rate was 1.0 mL/min and the detection wavelength was 327 nm.
Results: HPLC fingerprint of Clerodendrum lindleyi was established and 21 common peaks from 11 batches of samples were found.
Conclusion: The method has good precision, stability and repeatability, which can provide reliable basis for quality evaluation of Clerodendrum lindleyi.