Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice

Biotechnol Lett. 2016 May;38(5):793-9. doi: 10.1007/s10529-016-2034-2. Epub 2016 Jan 13.

Abstract

Objective: An oral lactococcal-based vaccine which haboured the haemagglutinin1 (HA1) antigen fused to nisP anchor protein for the purpose of surface displaying the HA1 antigen was developed against H1N1 virus.

Results: Recombinant L. lactis strains expressed HA1-nisP fusion proteins when induced with nisin, as confirmed through western blotting. However, immunofluorescense did not detect any surface-displayed proteins, suggesting that the protein was either unsuccessfully translocated or improperly displayed. Despite this, oral administration of recombinant L. lactis strains to BALB/c mice revealed that significant levels of anti-HA1 sIgA antibodies were detected in mice fecal suspension samples of mice group NZ9000 (pNZ:HN) when compared to the negative control NZ9000 (pNZ8048) group.

Conclusion: Specific anti-HA1 sIgA antibodies were locally produced and live recombinant lactococcal vaccine was able to elicit humoral response of BALB/c mice despite unsuccessful surface display of the HA1 epitope.

Keywords: H1N1; Haemagglutinin; Influenza virus; Lactococcus lactis; Oral vaccine; Surface display; Vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Antibodies, Viral / analysis
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Cell Surface Display Techniques*
  • Genetic Vectors*
  • Hemagglutinin Glycoproteins, Influenza Virus / genetics
  • Hemagglutinin Glycoproteins, Influenza Virus / immunology*
  • Immunoglobulin A, Secretory / analysis*
  • Influenza Vaccines / administration & dosage
  • Influenza Vaccines / genetics
  • Influenza Vaccines / immunology*
  • Lactococcus lactis / genetics
  • Lactococcus lactis / growth & development*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mice, Inbred BALB C
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Subtilisins / genetics
  • Subtilisins / metabolism*

Substances

  • Antibodies, Viral
  • Bacterial Proteins
  • H1N1 virus hemagglutinin
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Immunoglobulin A, Secretory
  • Influenza Vaccines
  • Membrane Proteins
  • Recombinant Fusion Proteins
  • NisP protein, Lactococcus lactis
  • Subtilisins