Antioxidant Potential and In Situ Analysis of Major and Trace Element Determination of Ood-saleeb, a Known Unani Herbal Medicine by ICP-MS

Biol Trace Elem Res. 2016 Aug;172(2):521-527. doi: 10.1007/s12011-015-0607-x. Epub 2016 Jan 13.

Abstract

The intention of the present research work was to investigate the antioxidant activity and trace element analysis of Ood-saleeb, a known herbal medicine. Preliminary screening of phytochemicals showed that the extract of Ood-saleeb had flavonoids and phenolics. The significant activities in all antioxidant assays were observed in the extract of Ood-saleeb in comparison with the standard antioxidant with respect to dose of Ood-saleeb. Incredible activities to scavenge reactive oxygen species were also observed by the extract of Ood-saleeb. The IC50 values of all factors were determined using ascorbic acid as a standard. The inductive coupled plasma-mass spectroscopy (ICP-MS) was employed for the estimation of trace elements in Ood-saleeb extract. The concentrations of up to 18 elements were detected successfully. Silicon was found in high concentration (85.3 μg/g) while lithium was in low concentration (3 ng/g). The trace elements in the sample were found at different percentage levels which play a key role in the treatment of diseases.

Keywords: Antioxidant; Ascorbic acid; ICP-MS; Ood-saleeb; Trace elements.

MeSH terms

  • Antioxidants / analysis*
  • Antioxidants / metabolism
  • Flavonoids / analysis
  • Herbal Medicine*
  • Mass Spectrometry
  • Paeonia / chemistry*
  • Phenols / analysis
  • Plant Roots / chemistry*
  • Plants, Medicinal / chemistry*
  • Reactive Oxygen Species / metabolism
  • Trace Elements / analysis*

Substances

  • Antioxidants
  • Flavonoids
  • Phenols
  • Reactive Oxygen Species
  • Trace Elements