The main aim of this study was to explore physical activity regarding mothers of preschool children and connections concerning different aspects of their physical activity (at work, during transport, in free time, while doing housework) with subjective quality of life. Another aim was to investigate factors that are obstacles of living a physically active life in this population. The participants of the study were 252 Croatian women, who have at least one preschool child older than three years. The World Health Organization Quality Of Life - BREF questionnaire and International Physical Activity Questionnaire were used. Participants also gave some personal demographic data, and answered to questions about obstacles for participation in organized physical activity. Results of this research showed that mothers of preschool children were more physically active than the population of adults in general, but their physical activity was mostly housework activity. Physical activity in their free time was the only aspect of physical activity that was significantly correlated with different aspects of mother's subjective quality of life. Employed mothers and those who live in the towns had more physical activities in their free time. Organizational factors and feeling that family suffers for their absence were the most frequently perceived barriers for organized physical activity participation. Results revealed that only the physical activity in their free time contributes to mother's quality of life. Mothers of preschool children mostly do housework physical activity, so support of the social community for physical activity in their free time in this population is recommended. Such activities should be especially directed to unemployed mothers and those who live in the villages.