Morphology and systematics of two freshwater urostylid ciliates, with description of a new species (Protista, Ciliophora, Hypotrichia)

Xuming Pan; Yangbo Fan; Feng Gao; Zijian Qiu; Saleh A Al-Farraj; Alan Warren; Chen Shao

doi:10.1016/j.ejop.2015.11.003

Morphology and systematics of two freshwater urostylid ciliates, with description of a new species (Protista, Ciliophora, Hypotrichia)

Eur J Protistol. 2016 Feb:52:73-84. doi: 10.1016/j.ejop.2015.11.003. Epub 2015 Nov 27.

Authors

Xuming Pan¹, Yangbo Fan², Feng Gao³, Zijian Qiu⁴, Saleh A Al-Farraj⁵, Alan Warren⁶, Chen Shao⁷

Affiliations

¹ College of Life Science and Technology, Harbin Normal University, Harbin 150025, China; Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
² Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
³ Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, China. Electronic address: gaof@ouc.edu.cn.
⁴ College of Life Science and Technology, Harbin Normal University, Harbin 150025, China.
⁵ Zoology Department, King Saud University, Riyadh 11451, Saudi Arabia.
⁶ Department of Life Sciences, Natural History Museum, London SW7 5BD, UK.
⁷ The Key Laboratory of Biomedical Information Engineering, Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: andrews1201@hotmail.com.

PMID: 26752607
DOI: 10.1016/j.ejop.2015.11.003

Abstract

The morphology of two freshwater urostylid species, Neourostylopsis flava spec. nov. and Pseudourostyla subtropicaChen et al., 2014, isolated from freshwater ponds in northern and southern China, respectively, was investigated following examination of specimens in vivo and following protargol staining. Neourostylopsis flava spec. nov. is distinguished from its congeners by the following characteristics: body size 150-220 × 50-75 μm in vivo; yellow in colour; bright yellow to yellow-brownish spherical cortical granules densely arranged along marginal cirral rows and in irregular short rows on dorsal side; adoral zone with 40-55 membranelles; six to eight frontal, three or four buccal, two pretransverse ventral and seven to nine transverse cirri; 27-40 midventral pairs extending to about anterior 55% of cell; four or five left and four right marginal rows; freshwater habitat. A redescription of a freshwater population of P. subtropica is also provided. Phylogenetic analyses based on small subunit ribosomal DNA sequences shows that P. subtropica and N. flava spec. nov. group with their congeners and both Neourostylopsis and Pseudourostyla are monophyletic.

Keywords: Freshwater ciliate; Morphology; Neourostylopsis flava spec. nov.; Phylogeny; Pseudourostyla subtropica; SSU rDNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

China
Ciliophora / classification*
Ciliophora / cytology*
Fresh Water / parasitology
RNA, Ribosomal, 18S / genetics
Species Specificity

Substances

RNA, Ribosomal, 18S