Asymmetric Mach-Zehnder Interferometer Based Biosensors for Aflatoxin M1 Detection

Biosensors (Basel). 2016 Jan 6;6(1):1. doi: 10.3390/bios6010001.

Abstract

In this work, we present a study of Aflatoxin M1 detection by photonic biosensors based on Si₃N₄ Asymmetric Mach-Zehnder Interferometer (aMZI) functionalized with antibodies fragments (Fab'). We measured a best volumetric sensitivity of 10⁴ rad/RIU, leading to a Limit of Detection below 5 × 10(-7) RIU. On sensors functionalized with Fab', we performed specific and non-specific sensing measurements at various toxin concentrations. Reproducibility of the measurements and re-usability of the sensor were also investigated.

Keywords: Aflatoxin M1; Fab′; Mach–Zehnder interferometer; biosensor; limit of detection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aflatoxin M1 / isolation & purification*
  • Biosensing Techniques / instrumentation*
  • Interferometry
  • Optical Phenomena
  • Photons
  • Reproducibility of Results
  • Silicon Compounds / chemistry*

Substances

  • Silicon Compounds
  • Aflatoxin M1
  • silicon nitride