Protein-templated cobaltous phosphate nanocomposites for the highly sensitive and selective detection of platelet-derived growth factor-BB

Abstract

We synthesized novel Co3(PO4)2-based nanocomposites with 3D porous architectures via self-assembly; here, bovine serum albumin (BSA) and aptamer were used as organic phases to produce Co3(PO4)2@BSA and Co3(PO4)2@Apt nanocomposites, respectively. The formation mechanism of Co3(PO4)2-based nanocomposites was described based on characterizations of their physio-chemical performance, and the developed nanocomposites were applied as scaffold materials to construct a novel electrochemical aptasensor and detect platelet-derived growth factor-BB (PDGF-BB). The PDGF-BB targeting aptamer must be immobilized onto the Co3(PO4)2@BSA-modified electrode to detect PDGF-BB, whereas Co3(PO4)2@Apt-based aptasensor may be directly used to determine the target protein. Electrochemical impedance spectroscopy results showed that the developed Co3(PO4)2@BSA- and Co3(PO4)2@Apt-based aptasensors present highly sensitive detection ability toward PDGF-BB. Due to the special nanoflower structure, the Co3(PO4)2@BSA-based aptasensor features a detection limit of 3.7 pg mL(-1); while the limit of detection of the Co3(PO4)2@Apt-based aptasensor is 61.5 pg mL(-1), which is the possible bioactivity loss of the aptamer in Co3(PO4)2@Apt nanocomposite. The two detection limits obtained are still much lower than or comparable with those of previously reported aptasensors. The Co3(PO4)2@BSA- and Co3(PO4)2@Apt-based aptasensors showed high selectivity, stability, and applicability for detecting the desired protein. This finding indicates that the Co3(PO4)2-based nanocomposites could be used as an electrochemical biosensor for various detection procedures in the biomedical field.

Keywords: Aptasensor; Cobaltous phosphate nanocomposites; Platelet-derived growth factor-BB.