Engineering Stem Cell Organoids

Xiaolei Yin; Benjamin E Mead; Helia Safaee; Robert Langer; Jeffrey M Karp; Oren Levy

doi:10.1016/j.stem.2015.12.005

Engineering Stem Cell Organoids

Cell Stem Cell. 2016 Jan 7;18(1):25-38. doi: 10.1016/j.stem.2015.12.005.

Authors

Xiaolei Yin¹, Benjamin E Mead¹, Helia Safaee², Robert Langer³, Jeffrey M Karp⁴, Oren Levy⁵

Affiliations

¹ Division of Biomedical Engineering, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Cambridge, MA 02115, USA; Harvard Medical School, Cambridge, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Harvard - MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
² Division of Biomedical Engineering, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Cambridge, MA 02115, USA; Harvard Medical School, Cambridge, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Harvard - MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA.
³ Harvard - MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
⁴ Division of Biomedical Engineering, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Cambridge, MA 02115, USA; Harvard Medical School, Cambridge, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Harvard - MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA. Electronic address: jeffkarp.bwh@gmail.com.
⁵ Division of Biomedical Engineering, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Cambridge, MA 02115, USA; Harvard Medical School, Cambridge, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Harvard - MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA. Electronic address: levyorn@gmail.com.

Abstract

Organoid systems leverage the self-organizing properties of stem cells to create diverse multi-cellular tissue proxies. Most organoid models only represent single or partial components of a tissue, and it is often difficult to control the cell type, organization, and cell-cell/cell-matrix interactions within these systems. Herein, we discuss basic approaches to generate stem cell-based organoids, their advantages and limitations, and how bioengineering strategies can be used to steer the cell composition and their 3D organization within organoids to further enhance their utility in research and therapies.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review

MeSH terms

Animals
Brain / metabolism
Cell Communication
Cell Culture Techniques*
Humans
Intercellular Signaling Peptides and Proteins / metabolism
Intestinal Mucosa / metabolism
Microfluidics
Organogenesis
Organoids / cytology*
Regeneration
Stem Cells / cytology*
Tissue Engineering / methods

Substances

Intercellular Signaling Peptides and Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding