Abstract
Leucine is known to increase mTOR-mediated phosphorylation of 4EBP. In this study, leucine was administered to skeletal muscle-PGC-1α knockout mice. We observed attenuated 4EBP phosphorylation in the skeletal muscle, but not in the liver, of the PGC-1α knockout mice. These data suggest that skeletal muscle-PGC-1α is important for leucine-mediated mTOR activation and protein biosynthesis.
Keywords:
4EBP; leucine; mTOR; muscle specific PGC-1α knockout mouse.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Administration, Oral
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Animals
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Cell Cycle Proteins
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Eukaryotic Initiation Factors
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Gene Expression Regulation
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Leucine / administration & dosage*
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Liver / drug effects
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Liver / metabolism
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Mice
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Mice, Knockout
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Muscle, Skeletal / drug effects*
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Muscle, Skeletal / metabolism
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Organ Specificity
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Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
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Phosphoproteins / genetics*
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Phosphoproteins / metabolism
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Phosphorylation
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Protein Biosynthesis / drug effects
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RNA, Messenger / genetics*
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RNA, Messenger / metabolism
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Signal Transduction
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TOR Serine-Threonine Kinases / genetics*
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TOR Serine-Threonine Kinases / metabolism
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Transcription Factors / deficiency
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Transcription Factors / genetics*
Substances
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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Cell Cycle Proteins
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Eif4ebp1 protein, mouse
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Eukaryotic Initiation Factors
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Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
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Phosphoproteins
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Ppargc1a protein, mouse
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RNA, Messenger
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Transcription Factors
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mTOR protein, mouse
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TOR Serine-Threonine Kinases
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Leucine