Synthesis and evaluations of an acid-cleavable, fluorescently labeled nucleotide as a reversible terminator for DNA sequencing

Chem Commun (Camb). 2016 Feb 11;52(12):2549-52. doi: 10.1039/c5cc09578f. Epub 2016 Jan 8.

Abstract

An acid-cleavable linker based on a dimethylketal moiety was synthesized and used to connect a nucleotide with a fluorophore to produce a 3'-OH unblocked nucleotide analogue as an excellent reversible terminator for DNA sequencing by synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acids / chemistry*
  • Fluorescence
  • Fluorescent Dyes / chemistry*
  • Nucleotides / chemistry*
  • Polymerization
  • Sequence Analysis, DNA*

Substances

  • Acids
  • Fluorescent Dyes
  • Nucleotides