Synthesis, characterization and antibacterial assessment of SiO2-hydroxypropylmethyl cellulose hybrid materials with embedded silver nanoparticles

Tsvetelina Angelova; Nadezhda Rangelova; Hristina Dineva; Nelly Georgieva; Rudolf Müller

doi:10.1080/13102818.2014.944789

Synthesis, characterization and antibacterial assessment of SiO₂-hydroxypropylmethyl cellulose hybrid materials with embedded silver nanoparticles

Biotechnol Biotechnol Equip. 2014 Jul 4;28(4):747-752. doi: 10.1080/13102818.2014.944789. Epub 2014 Sep 23.

Authors

Tsvetelina Angelova¹, Nadezhda Rangelova², Hristina Dineva¹, Nelly Georgieva¹, Rudolf Müller³

Affiliations

¹ Department of Biotechnology, University of Chemical Technology and Metallurgy , Sofia , Bulgaria.
² Department of Fundamentals of Chemical Technology, University of Chemical Technology and Metallurgy , Sofia , Bulgaria.
³ Institute of Technical Biocatalysis, Hamburg University of Technology , Hamburg , Germany.

Abstract

Antibacterial SiO₂ hybrid materials based on tetraethyl orthosilicate (TEOS), hydroxypropylmethylcellulose (HPMC) and silver were prepared by the sol-gel method. The content of cellulose derivate was 5 wt% and the silver concentration varied from 0.5 wt% to 2.5 wt%. The amorphous nature, morphology and antibacterial behaviour were studied. Fourier transform infrared (FTIR) spectra of the hybrids showed characteristic peaks for SiO₂ network. Scanning electron microscope (SEM) analysis confirmed the formation of spherically shaped silver nanoparticles with a size of 30 nm on the matrix surfaces. Bacillus subtilis and Escherichia coli K12 were used as model microorganisms. The hybrid materials demonstrated bacteriostatic and bactericidal effect on the tested bacteria. Highest sensitivity to the obtained hybrids was observed in B. subtilis with significant lag-phase delay and biggest inhibition zone sizes.

Keywords: SiO2; antibacterial activity; hydroxypropylmethyl cellulose; silver nanoparticles.

Grants and funding

This work was supported by the European Social Fund, Operational Programme Human Resources Development (2007–2013) and the Bulgarian Ministry of Education and Science [grant number BG051PO001-3.3.06-0059].