Cyclic dinucleotide detection with riboswitch-G-quadruplex hybrid

Genichiro Tsuji; Herman O Sintim

doi:10.1039/c5mb00751h

Cyclic dinucleotide detection with riboswitch-G-quadruplex hybrid

Mol Biosyst. 2016 Mar;12(3):773-7. doi: 10.1039/c5mb00751h.

Authors

Genichiro Tsuji¹, Herman O Sintim²

Affiliations

¹ Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
² Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA and Center for Drug Discovery, Purdue University, West Lafayette, IN 47907, USA. hsintim@purdue.edu.

PMID: 26739090
DOI: 10.1039/c5mb00751h

Abstract

A cyclic dinucleotide riboswitch has been fused with a G-quadruplex motif to produce a conditional riboswitch-peroxidase-mimicking sensor that oxidizes both colorimetric and fluorogenic substrates in the presence of c-di-GMP. We find that signal-to-noise ratio could be improved by using a two-, not three-, floor split G-quadruplex for this conditional peroxidase-mimicking riboswitch.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Base Sequence
Cyclic GMP / analogs & derivatives*
Cyclic GMP / analysis
Dimerization
Fluorescence
G-Quadruplexes*
Molecular Sequence Data
Nucleic Acid Heteroduplexes / chemistry*
Oxidation-Reduction
Peroxidase / metabolism
Riboswitch*
Tyramine

Substances

Nucleic Acid Heteroduplexes
Riboswitch
bis(3',5')-cyclic diguanylic acid
Peroxidase
Cyclic GMP
Tyramine