Abstract
DNA segments encoding signal peptides from mouse alpha-amylase, yeast acid phosphatase, and yeast invertase were fused in frame to a barley (1-3,1-4)-beta-glucanase cDNA gene and expressed in yeast cells under the control of the phosphoglycerate kinase gene promoter. Pure beta-glucanase is obtained by gel filtration of concentrated yeast cell supernatant. It was shown that the glucanase pre-protein was specifically processed and the mature protein efficiently secreted when the yeast invertase signal sequence directed secretion.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acid Phosphatase / genetics
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Amino Acid Sequence
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Animals
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Base Sequence
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Genes*
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Genes, Fungal
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Glycoside Hydrolases / genetics*
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Glycoside Hydrolases / metabolism
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Hordeum / enzymology
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Hordeum / genetics
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Mice
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Molecular Sequence Data
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Oligonucleotide Probes
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Plants / enzymology
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Plants / genetics*
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Plasmids
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Promoter Regions, Genetic
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Protein Conformation
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Protein Sorting Signals / genetics
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Recombinant Proteins / metabolism
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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alpha-Amylases / genetics
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beta-Fructofuranosidase
Substances
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Oligonucleotide Probes
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Protein Sorting Signals
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Recombinant Proteins
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Acid Phosphatase
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Glycoside Hydrolases
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alpha-Amylases
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beta-Fructofuranosidase
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licheninase