Photo-click construction of a targetable and activatable two-photon probe imaging protease in apoptosis

Mi Zhou; Jing Hu; Mengmeng Zheng; Qinhua Song; Jinbo Li; Yan Zhang

doi:10.1039/c5cc09973k

Photo-click construction of a targetable and activatable two-photon probe imaging protease in apoptosis

Chem Commun (Camb). 2016 Feb 7;52(11):2342-5. doi: 10.1039/c5cc09973k.

Authors

Mi Zhou¹, Jing Hu¹, Mengmeng Zheng¹, Qinhua Song², Jinbo Li¹, Yan Zhang¹

Affiliations

¹ State Key Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry & BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China. njuzy@nju.edu.cn jinboli@nju.edu.cn.
² Department of Chemistry, Joint Laboratory of Green Synthetic Chemistry, University of Science and Technology of China, Hefei 230026, P. R. China.

PMID: 26729240
DOI: 10.1039/c5cc09973k

Abstract

A photo-click reaction was used as an efficient method to construct two-photon fluorescent probes bearing two functional peptides for targeting and for protease cleavage respectively. The activatable two-photon probe constructed by this method was applied to two-photon imaging of caspase-3 both in cellular apoptosis and in tumor tissue.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis*
Click Chemistry*
HeLa Cells
Humans
Peptide Hydrolases / therapeutic use*
Photons*

Substances

Peptide Hydrolases