Detection of Long Noncoding RNA Expression by Nonradioactive Northern Blots

Methods Mol Biol. 2016:1402:177-188. doi: 10.1007/978-1-4939-3378-5_14.

Abstract

With the advances in sequencing technology and transcriptome analysis, it is estimated that up to 75 % of the human genome is transcribed into RNAs. This finding prompted intensive investigations on the biological functions of noncoding RNAs and led to very exciting discoveries of microRNAs as important players in disease pathogenesis and therapeutic applications. Research on long noncoding RNAs (lncRNAs) is in its infancy, yet a broad spectrum of biological regulations has been attributed to lncRNAs. As a novel class of RNA transcripts, the expression level and splicing variants of lncRNAs are various. Northern blot analysis can help us learn about the identity, size, and abundance of lncRNAs. Here we describe how to use northern blot to determine lncRNA abundance and identify different splicing variants of a given lncRNA.

Keywords: Long noncoding RNA; Northern blots; RNA expression.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern / methods*
  • Gene Expression
  • Humans
  • Nucleic Acid Hybridization / methods
  • Nucleic Acid Probes / analysis
  • Nucleic Acid Probes / genetics
  • RNA Splicing
  • RNA, Long Noncoding / analysis*
  • RNA, Long Noncoding / genetics

Substances

  • Nucleic Acid Probes
  • RNA, Long Noncoding