Profiling Long Noncoding RNA Expression Using Custom-Designed Microarray

Xinna Zhang; Gabriel Lopez-Berestein; Anil K Sood; George A Calin

doi:10.1007/978-1-4939-3378-5_5

Profiling Long Noncoding RNA Expression Using Custom-Designed Microarray

Methods Mol Biol. 2016:1402:33-41. doi: 10.1007/978-1-4939-3378-5_5.

Authors

Xinna Zhang^{1

2}, Gabriel Lopez-Berestein^{3

4

4}, Anil K Sood^{5

6

4}, George A Calin^{7

8

9}

Affiliations

¹ Center for RNA Interference and Non-coding RNAs, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX, 77030, USA. xzhang12@mdanderson.org.
² Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. xzhang12@mdanderson.org.
³ Center for RNA Interference andNon-coding RNAs, The University of Texas MD Anderson Cancer Center (MDACC), 1515 Holcombe Blvd., Houston, TX, 77030, USA.
⁴ Department of Cancer Biology, The University of Texas MD Anderson Cancer Center (MDACC), Houston, TX, USA.
⁵ Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center (MDACC), 1515 Holcombe Blvd., Houston, TX, 77030, USA.
⁶ Center for RNA Interference and Non-Coding RNAs, The University of Texas MD Anderson Cancer Center (MDACC), Houston, TX, USA.
⁷ Center for RNA Interference and Non-Coding RNAs, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
⁸ Department of GynecologicOncology and ReproductiveMedicine, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX, 77030, USA.
⁹ Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

PMID: 26721482
DOI: 10.1007/978-1-4939-3378-5_5

Abstract

Long noncoding RNAs (lncRNAs) are an important class of pervasive genes involved in a variety of biological functions. The abnormal expression of lncRNAs has been implicated in a range of many human diseases, including cancer. But only a small number of functional lncRNAs have been well characterized to date. lncRNA expression profiling may help to identify useful molecular biomarkers and targets for novel therapeutic approaches in the future. In this chapter, we describe a highly efficient lncRNA expression profiling method using a custom-designed microarray.

Keywords: Microarray profiling; cDNA labeling; cDNA synthesis; lncRNA expression.

MeSH terms

Base Sequence
DNA, Complementary / genetics
Gene Expression Profiling / instrumentation*
Humans
Oligonucleotide Array Sequence Analysis / instrumentation*
Polyadenylation
RNA, Long Noncoding / genetics*

Substances

DNA, Complementary
RNA, Long Noncoding