Preparation of a novel ionic hybrid stationary phase by non-covalent functionalization of single-walled carbon nanotubes with amino-derivatized silica gel for fast HPLC separation of aromatic compounds

Hayriye Aral; K Serdar Çelik; Tarık Aral; Giray Topal

doi:10.1016/j.talanta.2015.11.029

Preparation of a novel ionic hybrid stationary phase by non-covalent functionalization of single-walled carbon nanotubes with amino-derivatized silica gel for fast HPLC separation of aromatic compounds

Talanta. 2016 Mar:149:21-29. doi: 10.1016/j.talanta.2015.11.029. Epub 2015 Nov 14.

Authors

Hayriye Aral¹, K Serdar Çelik², Tarık Aral², Giray Topal³

Affiliations

¹ University of Batman, Faculty of Science and Art, Department of Chemistry, Batman, Turkey. Electronic address: hayriye.aral@batman.edu.tr.
² University of Batman, Faculty of Science and Art, Department of Chemistry, Batman, Turkey.
³ University of Dicle, Faculty of Education, Department of Chemistry, Diyarbakır, Turkey.

PMID: 26717810
DOI: 10.1016/j.talanta.2015.11.029

Abstract

Single-walled carbon nanotubes (SWCNTs) were immobilized on spherical silica gel with a 4-μm average particle size and a 60-Å average pore size. The amino-derivatized silica gel was non-covalently coated with carboxylated SWCNTs to preserve the structure of the nanotubes and their physico-chemical properties. The novel ionic hybrid stationary phase was characterized by scanning electron microscopy (SEM), infra-red (IR) spectroscopy and elemental analysis, and then, it was used to fill an empty 150×4.6mm(2) high-performance liquid chromatography (HPLC) column. Chromatographic parameters, such as the theoretical plate number, retention factor and peak asymmetry factor, and analytical parameters, such as the limit of detection (LOD), limit of quantification (LOQ), linear range, calibration equation, and R(2) value, and quantitative analysis parameters were calculated for all of the analytes. Using different mobile phases, five different classes of aromatic hydrocarbons were separated in a very short analysis time of 4-8min. Furthermore, a high theoretical plate number (up to 25000) and an excellent peak asymmetry factor (1.0) were obtained. The results showed that the surface of the SWNTs had very strong interactions with aromatic groups, therefore providing high selectivity for the separation of different classes of aromatic compounds. This study indicates that SWCNTs enable the extension of the application range of the newly prepared stationary phases for the fast separation of aromatic compounds by HPLC.

Keywords: Aromatic compound; Carbon nanotube; Fast separation; HPLC; Hybrid stationary phase; SWCNT.