Identification and Evaluation of Antiepileptic Activity of C21 Steroidal Glycosides from the Roots of Cynanchum wilfordii

J Nat Prod. 2016 Jan 22;79(1):89-97. doi: 10.1021/acs.jnatprod.5b00766. Epub 2015 Dec 30.

Abstract

Nine new C21 steroidal glycosides, named cynawilfosides A-I (1-9), along with 12 known compounds were isolated from the roots of Cynanchum wilfordii. The structures of the new compounds were elucidated by spectroscopic analysis and chemical methods. The five major components, cynawilfoside A (1), cynauricoside A (11), wilfoside C1N (16), wilfoside K1N (17), and cyanoauriculoside G (18), exhibited significant protection activity in a maximal electroshock (MES)-induced mouse seizure model with ED50 values of 48.5, 95.3, 124.1, 72.3, and 88.1 mg/kg, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anticonvulsants / chemistry
  • Anticonvulsants / isolation & purification*
  • Anticonvulsants / pharmacology*
  • Cynanchum / chemistry*
  • Disease Models, Animal
  • Glycosides / chemistry
  • Glycosides / isolation & purification*
  • Glycosides / pharmacology*
  • Mice
  • Molecular Structure
  • Plant Roots / chemistry
  • Pregnanes / chemistry
  • Pregnanes / isolation & purification*
  • Pregnanes / pharmacology*
  • Saponins
  • Seizures

Substances

  • Anticonvulsants
  • Glycosides
  • Pregnanes
  • Saponins
  • wilfoside C1N