Designing anthraquinone-pyrrole redox intercalating probes for electrochemical gene detection

Yu-Jen Lin; Yung-Chao Wu; Veerappan Mani; Sheng-Tung Huang; Chih-Hung Huang; Yi-Chiuen Hu; His-Chi Peter Shan

doi:10.1016/j.bios.2015.12.042

Designing anthraquinone-pyrrole redox intercalating probes for electrochemical gene detection

Biosens Bioelectron. 2016 May 15:79:294-9. doi: 10.1016/j.bios.2015.12.042. Epub 2015 Dec 17.

Authors

Yu-Jen Lin¹, Yung-Chao Wu², Veerappan Mani³, Sheng-Tung Huang⁴, Chih-Hung Huang², Yi-Chiuen Hu⁵, His-Chi Peter Shan⁶

Affiliations

¹ Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan, ROC.
² Graduate Institute of Biomedical and Biochemical Engineering, National Taipei University of Technology, Taipei, Taiwan, ROC.
³ Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan, ROC; Graduate Institute of Biomedical and Biochemical Engineering, National Taipei University of Technology, Taipei, Taiwan, ROC.
⁴ Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan, ROC; Graduate Institute of Biomedical and Biochemical Engineering, National Taipei University of Technology, Taipei, Taiwan, ROC. Electronic address: ws75624@ntut.edu.tw.
⁵ National Applied Research Lab, Hsinchu, Taiwan, ROC.
⁶ WinSense Biotech Corporation, Hsinchu, Taiwan, ROC.

PMID: 26716423
DOI: 10.1016/j.bios.2015.12.042

Abstract

The real-time quantitative electrochemical monitoring of nucleic acid amplification through PCR is a promising renowned methodology to detect pathogenic DNAs. In this work, anthraquinone-pyrrole derivatives based redox intercalating probes (AP probes: AP1, AP2) have been designed, synthesized, characterized and successfully demonstrated in real-time like quantitative PCR. The rationally designed AP probes exhibited excellent DNA binding abilities and electrochemical behaviors. The binding parameters such as binding constant, binding site size and diffusion coefficient were estimated which were comparable to literature reports. Besides, the AP probes are highly stable under PCR thermal conditions and did not inhibit PCR. Therefore, a real-time like quantification of DNA amplification was demonstrated to quantify the initial copy number of target genes. The probe AP2 has excellent ability to detect ~10(3) copies of target tpc DNA with good sensitivity. The AP probes are metal-free, easily synthesizable, non-toxic, thermally stable and feasible for miniaturized PCR chips.

Keywords: Biosensor; DNA amplification; DNA sensor; Electrochemistry; Molecular design; Polymerase chain reaction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anthraquinones / chemistry
Biosensing Techniques / methods*
DNA / chemistry
DNA / isolation & purification*
Oxidation-Reduction
Polymerase Chain Reaction*
Pyrroles / chemistry

Substances

Anthraquinones
Pyrroles
DNA