Microarray-based gene expression analysis of strong seed dormancy in rice cv. N22 and less dormant mutant derivatives

Tao Wu; Chunyan Yang; Baoxu Ding; Zhiming Feng; Qian Wang; Jun He; Jianhua Tong; Langtao Xiao; Ling Jiang; Jianmin Wan

doi:10.1016/j.plaphy.2015.12.001

Microarray-based gene expression analysis of strong seed dormancy in rice cv. N22 and less dormant mutant derivatives

Plant Physiol Biochem. 2016 Feb:99:27-38. doi: 10.1016/j.plaphy.2015.12.001. Epub 2015 Dec 9.

Authors

Tao Wu¹, Chunyan Yang¹, Baoxu Ding¹, Zhiming Feng¹, Qian Wang¹, Jun He¹, Jianhua Tong², Langtao Xiao², Ling Jiang³, Jianmin Wan⁴

Affiliations

¹ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China.
² Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha, 410128, China.
³ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: jiangling@njau.edu.cn.
⁴ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

PMID: 26713549
DOI: 10.1016/j.plaphy.2015.12.001

Abstract

Seed dormancy in rice is an important trait related to the pre-harvest sprouting resistance. In order to understand the molecular mechanisms of seed dormancy, gene expression was investigated by transcriptome analysis using seeds of the strongly dormant cultivar N22 and its less dormant mutants Q4359 and Q4646 at 24 days after heading (DAH). Microarray data revealed more differentially expressed genes in Q4359 than in Q4646 compared to N22. Most genes differing between Q4646 and N22 also differed between Q4359 and N22. GO analysis of genes differentially expressed in both Q4359 and Q4646 revealed that some genes such as those for starch biosynthesis were repressed, whereas metabolic genes such as those for carbohydrate metabolism were enhanced in Q4359 and Q4646 seeds relative to N22. Expression of some genes involved in cell redox homeostasis and chromatin remodeling differed significantly only between Q4359 and N22. The results suggested a close correlation between cell redox homeostasis, chromatin remodeling and seed dormancy. In addition, some genes involved in ABA signaling were down-regulated, and several genes involved in GA biosynthesis and signaling were up-regulated. These observations suggest that reduced seed dormancy in Q4359 was regulated by ABA-GA antagonism. A few differentially expressed genes were located in the regions containing qSdn-1 and qSdn-5 suggesting that they could be candidate genes underlying seed dormancy. Our work provides useful leads to further determine the underling mechanisms of seed dormancy and for cloning seed dormancy genes from N22.

Keywords: ABA-GA; Germination; Microarray; Oryza sativa; Seed dormancy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abscisic Acid / metabolism
Gene Expression Regulation, Plant*
Gibberellins / metabolism
Microarray Analysis
Mutation
Oryza / genetics*
Oryza / metabolism*
Plant Dormancy / genetics*
Seeds / genetics
Seeds / metabolism*

Substances

Gibberellins
Abscisic Acid