Objective: To investigate the anticancer effect and its mechanism of SN-38 combined with sorafenib on hepatocellular cancer cell lines HepG-2 and BEL-7402.
Methods: SRB colorimetry was employed to measure the viability of HepG-2 and BEL-7402 cells after the treatment of SN-38 with sorafenib. Propidium iodide flow cytometric assay and DAPI staining were used to evaluate the apoptosis of HCC cells. Western blotting was conducted to detect the expression level of apoptosis-related and DNA damage-related proteins.
Results: SRB colorimetry showed the synergistic anticancer activities of SN-38 combined with sorafenib, with a combination index of <0.9. The apoptotic rates of HepG-2 cells in control, 60 nmol/L SN-38, 2.5μmol/L sorafenib and combination groups were 4.25%±2.45%, 28.95%±10.75%, 3.49%±2.49% and 53.19%±11.21%, respectively(P<0.05). Western blotting showed that the combination of these two drugs increased the enzymolysis of PARP, Caspase-8 and Caspase-3, and promoted the expression levels of p53, p21 and γ-H2AX significantly.
Conclusion: SN-38 and sorafenib have synergistic anticancer activity on hepatocellular carcinoma cells in vitro with the augmentation of apoptosis.
目的: 研究伊立替康的活性代谢物SN-38与索拉非尼联合作用于HepG-2和BEL-7402细胞株的抗肝癌效果及其相关机制。
方法: 利用磺酰罗丹明B显色法测定SN-38与索拉非尼单用或合用后HepG-2和BEL-7402细胞的存活率。利用PI染色结合流式细胞术及DAPI染色法检测细胞凋亡, 同时利用蛋白质印迹法检测凋亡相关蛋白、DNA损伤标志蛋白的表达情况。
结果: 与单药比较, SN-38与索拉非尼合用对细胞的抑制作用增强, 合用指数小于0.9。对照组、SN-38组、索拉非尼组、合用组HepG-2细胞凋亡率分别为4.25%±2.45%、28.95%±10.75%、3.49%±2.49%、53.19%±11.21%, 合用组细胞凋亡率增加(与其他组比较均 P < 0.05)。同时合用组凋亡相关蛋白多聚二磷酸腺苷核糖聚合酶(PARP)、半胱氨酸天冬氨酸蛋白酶(Caspase)8、Caspase-3的蛋白酶切量以及p53蛋白、p21蛋白、DNA损伤标志蛋白磷酸化的组蛋白H2AX的表达量均增加。
结论: 在细胞水平上, SN-38与索拉非尼联合应用能够通过p53表达增加促进肝癌细胞凋亡, 因此具有抗肝癌效果。