Preliminary investigations into surface molecularly imprinted nanoparticles for Helicobacter pylori eradication

Jiaying Han; Yinjing Sun; Jiapeng Hou; Yuyan Wang; Yu Liu; Cao Xie; Weiyue Lu; Jun Pan

doi:10.1016/j.apsb.2015.09.003

Preliminary investigations into surface molecularly imprinted nanoparticles for Helicobacter pylori eradication

Acta Pharm Sin B. 2015 Nov;5(6):577-82. doi: 10.1016/j.apsb.2015.09.003. Epub 2015 Oct 20.

Authors

Jiaying Han¹, Yinjing Sun¹, Jiapeng Hou¹, Yuyan Wang², Yu Liu¹, Cao Xie¹, Weiyue Lu¹, Jun Pan¹

Affiliations

¹ Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai 201203, China ; Key Laboratory of Smart Drug Delivery (Fudan University), Ministry of Education, Shanghai 201203, China.
² Department of Microbiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.

Abstract

This paper reports investigations into the preparation and characterization of surface molecularly imprinted nanoparticles (SMINs) designed to adhere to Helicobacter pylori (H. pylori). Imprinted nanoparticles were prepared by the inverse microemulsion polymerization method. A fraction of Lpp20, an outer membrane protein of H. pylori known as NQA, was chosen as template and modified with myristic acid to facilitate its localization on the surface of the nanoparticles. The interaction between these SMINs with the template NQA were evaluated using surface plasmon resonance (SPR), change in zeta potential and fluorescence polarization (FP). The results were highly consistent in demonstrating a preferential recognition of the template NQA for SMINs compared with the control nanoparticles. In vitro experiments also indicate that such SMINs are able to adhere to H. pylori and may be useful for H. pylori eradication.

Keywords: Epitope imprinting; Helicobacter pylori binding; Lpp20; NQA; Surface molecularly imprinted nanoparticles.