Interrogating Circulating Microsomes and Exosomes Using Metal Nanoparticles

Yi-Ge Zhou; Reza M Mohamadi; Mahla Poudineh; Leyla Kermanshah; Sharif Ahmed; Tina Saberi Safaei; Jessica Stojcic; Robert K Nam; Edward H Sargent; Shana O Kelley

doi:10.1002/smll.201502365

Interrogating Circulating Microsomes and Exosomes Using Metal Nanoparticles

Small. 2016 Feb 10;12(6):727-32. doi: 10.1002/smll.201502365. Epub 2015 Dec 28.

Authors

Affiliations

¹ Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada.
² Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON, M5S 3G4, Canada.
³ Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3M2, Canada.
⁴ Division of Urology, Sunnybrook Research Institute, University of Toronto, Toronto, ON, M4M 3M5, Canada.
⁵ Department of Biochemistry, University of Toronto, Toronto, ON, M5S 3M2, Canada.

PMID: 26707703
DOI: 10.1002/smll.201502365

Abstract

A chip-based approach for electrochemical characterization and detection of microsomes and exosomes based on direct electro-oxidation of metal nanoparticles (MNPs) that specifically recognize surface markers of these vesicles is reported. It is found that exosomes and microsomes derived from prostate cancer cells can be identified by their surface proteins EpCAM and PSMA, suggesting the potential of exosomes and microsomes for use as diagnostic biomarkers.

Keywords: electrochemistry; exosomes; microsomes; nanoparticles; prostate cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Electrochemistry
Exosomes / metabolism*
Exosomes / ultrastructure
Humans
Male
Metal Nanoparticles / chemistry*
Microsomes / metabolism*
Microsomes / ultrastructure
Prostatic Neoplasms / blood

Grants and funding

Canadian Institutes of Health Research/Canada