Real-time PCR validation of visually identified snapper Chrysophrys auratus (Sparidae) eggs

P J Dias; C B Wakefield; D V Fairclough; G Jackson; M J Travers; M Snow

doi:10.1111/jfb.12864

Real-time PCR validation of visually identified snapper Chrysophrys auratus (Sparidae) eggs

J Fish Biol. 2016 Feb;88(2):811-9. doi: 10.1111/jfb.12864. Epub 2015 Dec 28.

Authors

P J Dias^{1

2}, C B Wakefield¹, D V Fairclough¹, G Jackson¹, M J Travers¹, M Snow¹

Affiliations

¹ Department of Fisheries, Government of Western Australia, Western Australia Fisheries and Marine Research Laboratories, P. O. Box 20, North Beach, WA 6920, Australia.
² School of Animal Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

PMID: 26707536
DOI: 10.1111/jfb.12864

Abstract

In this study, a total of 212 eggs were visually identified as snapper Chrysophrys auratus. Real-time PCR confirmed visual identification in 69% of cases but corroboration varied widely among plankton samples. The use of molecular tools to support visual identification prior to adopting daily egg production stock assessment methods should be considered.

Keywords: Pagrus; daily egg production method; fisheries; spawning aggregation; spawning stock biomass.

MeSH terms

Animals
Australia
Biomass
Ovum / classification*
Perciformes / classification*
Perciformes / genetics
Real-Time Polymerase Chain Reaction*
Sequence Analysis, DNA