Anomalous redispersibility behavior of glycerophosphate deyhydrogenase microparticles dried in an acoustic levitator or bench-top spray dryer

Elke Lorenzen; Geoffrey Lee

doi:10.1016/j.ijpharm.2015.12.039

Anomalous redispersibility behavior of glycerophosphate deyhydrogenase microparticles dried in an acoustic levitator or bench-top spray dryer

Int J Pharm. 2016 Feb 10;498(1-2):316-7. doi: 10.1016/j.ijpharm.2015.12.039. Epub 2015 Dec 18.

Authors

Elke Lorenzen¹, Geoffrey Lee²

Affiliations

¹ Division of Pharmaceutics, Friedrich-Alexander University, Erlangen, Germany.
² Division of Pharmaceutics, Friedrich-Alexander University, Erlangen, Germany. Electronic address: geoff.lee@fau.de.

PMID: 26707244
DOI: 10.1016/j.ijpharm.2015.12.039

Abstract

The enzyme glycerophosphate dehydrogenase (GPDH) behaves differently when dried either as single droplets in an acoustic levitator or spray dried on a bench-top machine. The GPDH in particles dried in the levitator at a drying gas temperature of 60°C could not be redispersed in water, whereas spray drying at an outlet temperature of 92°C produced denaturation but the particles were redissolvable. One difference between the two processes is that the larger levitated droplets take longer to dry than the small spray dried droplets. The slow drying process of the levitated droplet/particle apparently causes denaturation that is sufficient to make the particles non-redispersible. This does not happen on spray drying.

Keywords: Acoustic levitator; Denature; Protein; Solubility; Spray dried.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acoustics
Chemistry, Pharmaceutical / methods*
Desiccation / methods*
Glycerolphosphate Dehydrogenase / analysis
Glycerolphosphate Dehydrogenase / chemistry*
Microspheres*

Substances

Glycerolphosphate Dehydrogenase