Disinfection of titanium dioxide nanotubes using super-oxidized water decrease bacterial viability without disrupting osteoblast behavior

Ernesto Beltrán-Partida; Benjamín Valdez-Salas; Alan Escamilla; Mario Curiel; Ernesto Valdez-Salas; Nicola Nedev; Jose M Bastidas

doi:10.1016/j.msec.2015.11.042

Disinfection of titanium dioxide nanotubes using super-oxidized water decrease bacterial viability without disrupting osteoblast behavior

Mater Sci Eng C Mater Biol Appl. 2016 Mar:60:239-245. doi: 10.1016/j.msec.2015.11.042. Epub 2015 Nov 17.

Authors

Ernesto Beltrán-Partida¹, Benjamín Valdez-Salas², Alan Escamilla³, Mario Curiel³, Ernesto Valdez-Salas⁴, Nicola Nedev³, Jose M Bastidas⁵

Affiliations

¹ Department of Biomaterials, Dental Materials and Tissue Engineering, Faculty of Dentistry Mexicali, Autonomous University of Baja California, Av. Zotoluca and Chinampas St., 21040 Mexicali, Baja California, Mexico; Department of Corrosion and Materials, Engineering Institute, Autonomous University of Baja California, Blvd. Benito Juarez and Normal St., 21280 Mexicali, Baja California, Mexico.
² Department of Corrosion and Materials, Engineering Institute, Autonomous University of Baja California, Blvd. Benito Juarez and Normal St., 21280 Mexicali, Baja California, Mexico. Electronic address: benval@uabc.edu.mx.
³ Department of Corrosion and Materials, Engineering Institute, Autonomous University of Baja California, Blvd. Benito Juarez and Normal St., 21280 Mexicali, Baja California, Mexico.
⁴ Ixchel Medical Centre, Av. Bravo y Obregón, 21000 Mexicali, Baja California, Mexico.
⁵ National Centre for Metallurgical Research, CSIC, Av. Gregorio del Amo 8, 28040 Madrid, Spain.

PMID: 26706527
DOI: 10.1016/j.msec.2015.11.042

Abstract

Amorphous titanium dioxide (TiO2) nanotubes (NTs) on Ti6Al4V alloy were synthesized by anodization using a commercially available super-oxidized water (SOW). The NT surfaces were sterilized by ultraviolet (UV) irradiation and disinfected using SOW. The adhesion and cellular morphology of pig periosteal osteoblast (PPO) cells and the behavior of Staphylococcus aureus (S. aureus) cultured on the sterilized and disinfected surfaces were investigated. A non-anodized Ti6Al4V disc sterilized by UV irradiation (without SOW) was used as control. The results of this study reveal that the adhesion, morphology and filopodia development of PPO cells in NTs are dramatically improved, suggesting that SOW cleaning may not disrupt the benefits obtained by NTs. Significantly decreased bacterial viability in NTs after cleaning with SOW and comparing with non-cleaned NTs was seen. The results suggest that UV and SOW could be a recommendable method for implant sterilization and disinfection without altering osteoblast behavior while decreasing bacterial viability.

Keywords: Bacterial viability; Nanostructures; Sterilization; Super-oxidized water; Titanium.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disinfection
Microbial Viability / drug effects
Nanostructures / chemistry*
Nanotubes / chemistry*
Osteoblasts / cytology*
Osteoblasts / drug effects
Staphylococcus aureus / drug effects
Surface Properties
Titanium / chemistry*
Titanium / pharmacology*

Substances

titanium dioxide
Titanium