Production of γ-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system

Yu Kiat Lin; Pau Loke Show; Yee Jiun Yap; Arbakariya B Ariff; Mohammad Suffian Mohammad Annuar; Oi Ming Lai; Teck Kim Tang; Joon Ching Juan; Tau Chuan Ling

doi:10.1016/j.jbiosc.2015.11.001

Production of γ-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system

J Biosci Bioeng. 2016 Jun;121(6):692-696. doi: 10.1016/j.jbiosc.2015.11.001. Epub 2015 Dec 17.

Authors

Yu Kiat Lin¹, Pau Loke Show², Yee Jiun Yap³, Arbakariya B Ariff⁴, Mohammad Suffian Mohammad Annuar¹, Oi Ming Lai⁵, Teck Kim Tang⁵, Joon Ching Juan⁶, Tau Chuan Ling⁷

Affiliations

¹ Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
² Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia; Manufacturing and Industrial Processes Division, Faculty of Engineering, Centre for Food and Bioproduct Processing, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia.
³ Department of Applied Mathematics, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia.
⁴ Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
⁵ Department of Bioprocess Technology, Faculty of Biotechnology and Bimolecular Sciences, University Putra Malaysia, 43400 Serdang, Malaysia.
⁶ Nanotechnology & Catalyst Research Centre (NANOCAT), University of Malaya, 50603 Kuala Lumpur, Malaysia.
⁷ Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: tcling@um.edu.my.

PMID: 26702953
DOI: 10.1016/j.jbiosc.2015.11.001

Abstract

Aqueous two-phase system (ATPS) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. Extractive bioconversion of gamma-cyclodextrin (γ-CD) from soluble starch with cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) enzyme derived from Bacillus cereus was evaluated using polyethylene glycol (PEG)/potassium phosphate based on ATPS. The optimum condition was attained in the ATPS constituted of 30.0% (w/w) PEG 3000 g/mol and 7.0% (w/w) potassium phosphate. A γ-CD concentration of 1.60 mg/mL with a 19% concentration ratio was recovered after 1 h bioconversion process. The γ-CD was mainly partitioned to the top phase (YT=81.88%), with CGTase partitioning in the salt-rich bottom phase (KCGTase=0.51). Repetitive batch processes of extractive bioconversion were successfully recycled three times, indicating that this is an environmental friendly and a cost saving technique for γ-CD production and purification.

Keywords: Aqueous two-phase system; Bacillus cereus; Cyclodextrin; Cyclodextrin glycosyltransferease; Extractive bioconversion.

MeSH terms

Bacillus cereus / enzymology*
Glucosyltransferases / metabolism*
Phosphates / chemistry*
Polyethylene Glycols / chemistry*
Potassium Compounds / chemistry*
Starch / chemistry
Starch / metabolism
Water / chemistry*
gamma-Cyclodextrins / isolation & purification*
gamma-Cyclodextrins / metabolism*

Substances

Phosphates
Potassium Compounds
gamma-Cyclodextrins
Water
Polyethylene Glycols
Starch
potassium phosphate
Glucosyltransferases
cyclomaltodextrin glucanotransferase
gamma-cyclodextrin