Picomolar detection of carcinoembryonic antigen in whole blood using microfluidics and surface-enhanced Raman spectroscopy

Kun Zou; Zhigang Gao; Quanfeng Deng; Yong Luo; Lijuan Zou; Yao Lu; Weijie Zhao; Bingcheng Lin

doi:10.1002/elps.201500535

Picomolar detection of carcinoembryonic antigen in whole blood using microfluidics and surface-enhanced Raman spectroscopy

Electrophoresis. 2016 Mar;37(5-6):786-9. doi: 10.1002/elps.201500535. Epub 2016 Jan 21.

Authors

Kun Zou¹, Zhigang Gao^{2

3}, Quanfeng Deng^{2

3}, Yong Luo^{2

3}, Lijuan Zou¹, Yao Lu⁴, Weijie Zhao^{2

3}, Bingcheng Lin⁴

Affiliations

¹ The Second Hospital of Dalian Medical University, Dalian, P. R. China.
² School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian, P. R. China.
³ State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian, P. R. China.
⁴ Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.

PMID: 26702874
DOI: 10.1002/elps.201500535

Abstract

Carcinoembryonic antigen (CEA) is a wide-spectrum biomarker. Clinically, we generally use serum sample to detect CEA, which needs to be centrifuged to pretreat the raw blood sample. In this study, we realized direct CEA detection in raw blood samples exploiting microfluidics. The LOD was as low as 10(-12) M.

Keywords: Carcinoembryonic antigen; Magnetic nanoparticles; Microfluidics; SERS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carcinoembryonic Antigen / blood*
Gold / chemistry
Limit of Detection
Magnetite Nanoparticles / chemistry
Microfluidic Analytical Techniques / methods*
Spectrum Analysis, Raman / methods*

Substances

Carcinoembryonic Antigen
Magnetite Nanoparticles
Gold