Multiplex-PCR for the detection of viruses in the CSF of infants and young children

J Med Virol. 2017 Mar;89(3):559-561. doi: 10.1002/jmv.24461. Epub 2016 Nov 10.

Abstract

We aimed to establish the utility of the Fast-track diagnostics Viral meningitis multiplex PCR kit for the diagnosis of central nervous system infection in infants. The multiplex assay had reduced sensitivity for the detection of enterovirus, the predominant pathogen in young infants, when compared to our in-house singleplex PCR. In our infant population, multiple singleplex PCR assays perform better than a multiplex assay for the detection of CSF viruses. J. Med. Virol. 89:559-561, 2017. © 2016 Wiley Periodicals, Inc.

Keywords: enterovirus; infants; multiplex PCR; viral meningitis.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Cerebrospinal Fluid / virology*
  • Female
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Meningitis, Viral / diagnosis*
  • Molecular Diagnostic Techniques / methods*
  • Multiplex Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Viruses / classification
  • Viruses / genetics
  • Viruses / isolation & purification*