Effects of methacholine infusion on desflurane pharmacokinetics in piglets

Alf Kozian; Moritz Kretzschmar; James E Baumgardner; Jens Schreiber; Göran Hedenstierna; Anders Larsson; Thomas Hachenberg; Thomas Schilling

doi:10.1016/j.dib.2015.11.002

Effects of methacholine infusion on desflurane pharmacokinetics in piglets

Data Brief. 2015 Nov 10:5:939-47. doi: 10.1016/j.dib.2015.11.002. eCollection 2015 Dec.

Authors

Alf Kozian¹, Moritz Kretzschmar¹, James E Baumgardner², Jens Schreiber³, Göran Hedenstierna⁴, Anders Larsson⁵, Thomas Hachenberg⁶, Thomas Schilling¹

Affiliations

¹ Department of Anesthesiology and Intensive Care Medicine, Otto-von-Guericke-University Magdeburg, Germany ; Department of Surgical Sciences, Hedenstierna Laboratory, Uppsala University, Uppsala, Sweden.
² Oscillogy ® LLC, Folsom, PA, USA.
³ Department of Pneumology, Otto-von-Guericke-University Magdeburg, Germany.
⁴ Department of Medical Sciences, Clinical Physiology, Uppsala University, Sweden.
⁵ Department of Surgical Sciences, Anesthesia and Intensive Care, Uppsala University, Uppsala, Sweden.
⁶ Department of Anesthesiology and Intensive Care Medicine, Otto-von-Guericke-University Magdeburg, Germany.

Abstract

The data of a corresponding animal experiment demonstrates that nebulized methacholine (MCh) induced severe bronchoconstriction and significant inhomogeneous ventilation and pulmonary perfusion (V̇A/Q̇) distribution in pigs, which is similar to findings in human asthma. The inhalation of MCh induced bronchoconstriction and delayed both uptake and elimination of desflurane (Kretzschmar et al., 2015) [1]. The objective of the present data is to determine V̇A/Q̇ matching by Multiple Inert Gas Elimination Technique (MIGET) in piglets before and during methacholine- (MCh-) induced bronchoconstriction, induced by MCh infusion, and to assess the blood concentration profiles for desflurane (DES) by Micropore Membrane Inlet Mass Spectrometry (MMIMS). Healthy piglets (n=4) under general anesthesia were instrumented with arterial, central venous, and pulmonary artery lines. The airway was secured via median tracheostomy with an endotracheal tube, and animals were mechanically ventilated with intermittent positive pressure ventilation (IPPV) with a FiO2 of 0.4, tidal volume (V T)=10 ml/kg and PEEP of 5cmH2O using an open system. The determination of V.A/Q. was done by MIGET: before desflurane application and at plateau in both healthy state and during MCh infusion. Arterial blood was sampled at 0, 1, 2, 5, 10, 20, and 30 min during wash-in and washout, respectively. Bronchoconstriction was established by MCH infusion aiming at doubling the peak airway pressure, after which wash-in and washout of the anesthetic gas was repeated. Anesthesia gas concentrations were measured by MMIMS. Data were analyzed by ANOVA, paired t-test, and by nonparametric Friedman׳s test and Wilcoxon׳s matched pairs test. We measured airway pressures, pulmonary resistance, and mean paO2 as well as hemodynamic variables in all pigs before desflurane application and at plateau in both healthy state and during methacholine administration by infusion. By MIGET, fractional alveolar ventilation and pulmonary perfusion in relation to the V.A/Q. compartments, data of logSDQ̇ and logSDV̇ (the second moments describing global dispersion, i.e. heterogeneity of distribution) were estimated prior to and after MCh infusion. The uptake and elimination of desflurane was determined by MMIMS.

Keywords: Alveolar ventilation; Animal model; Arterial blood gas concentration; Bronchoconstriction; Desflurane; Methacholine; Ventilation-/perfusion-mismatch.