Profile of Inflammation-associated genes during Hepatic Differentiation of Human Pluripotent Stem Cells

Joseph Ignatius Irudayam; Deisy Contreras; Lindsay Spurka; Songyang Ren; Vidhya Kanagavel; Arunachalam Ramaiah; Alagappan Annamalai; Samuel W French; Andrew S Klein; Vincent Funari; Vaithilingaraja Arumugaswami

doi:10.1016/j.dib.2015.10.023

Profile of Inflammation-associated genes during Hepatic Differentiation of Human Pluripotent Stem Cells

Data Brief. 2015 Nov 9:5:871-8. doi: 10.1016/j.dib.2015.10.023. eCollection 2015 Dec.

Affiliations

¹ Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
² Cedars-Sinai Genomics Core, Medical Genetics Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
³ Centre for Infectious Disease Research, Indian Institute of Science, Bangalore, Karnataka 560012, India ; Hindustan Genomics Institute, SVA Medical Center, Kadayam, Tamil Nadu 627415, India.
⁴ Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
⁵ Department of Pathology and Laboratory Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA.
⁶ Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA ; Department of Surgery, University of California at Los Angeles, Los Angeles, CA 90095, USA.
⁷ Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA ; Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA ; Department of Surgery, University of California at Los Angeles, Los Angeles, CA 90095, USA.

Abstract

Expression of genes associated with inflammation was analyzed during differentiation of human pluripotent stem cells (PSCs) to hepatic cells. Messenger RNA transcript profiles of differentiated endoderm (day 5), hepatoblast (day 15) and hepatocyte-like cells (day 21) were obtained by RNA sequencing analysis. When compared to endoderm cells an immature cell type, the hepatic cells (days 15 and 21) had significantly higher expression of acute phase protein genes including complement factors, coagulation factors, serum amyloid A and serpins. Furthermore, hepatic phase of cells expressed proinflammatory cytokines IL18 and IL32 as well as cytokine receptors IL18R1, IL1R1, IL1RAP, IL2RG, IL6R, IL6ST and IL10RB. These cells also produced CCL14, CCL15, and CXCL- 1, 2, 3, 16 and 17 chemokines. Endoderm cells had higher levels of chemokine receptors, CXCR4 and CXCR7, than that of hepatic cells. Sirtuin family of genes involved in aging, inflammation and metabolism were differentially regulated in endoderm and hepatic phase cells. Ligands and receptors of the tumor necrosis factor (TNF) family as well as downstream signaling factors TRAF2, TRAF4, FADD, NFKB1 and NFKBIB were differentially expressed during hepatic differentiation.

Keywords: SIRT1; cytokines; differential gene expression; differentiation; endoderm; hepatic cells; hepatoblast; hepatocytes; inflammation; pluripotent stem cells; sirtuin.

Profile of Inflammation-associated genes during Hepatic Differentiation of Human Pluripotent Stem Cells

Authors

Affiliations

Abstract

Grants and funding