Tissue MALDI Mass Spectrometry Imaging (MALDI MSI) of Peptides

Birte Beine; Hanna C Diehl; Helmut E Meyer; Corinna Henkel

doi:10.1007/978-1-4939-3341-9_10

Tissue MALDI Mass Spectrometry Imaging (MALDI MSI) of Peptides

Methods Mol Biol. 2016:1394:129-150. doi: 10.1007/978-1-4939-3341-9_10.

Authors

Birte Beine¹, Hanna C Diehl², Helmut E Meyer¹, Corinna Henkel³

Affiliations

¹ Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Dortmund, Germany.
² Clinical Proteomics, Medizinisches Proteom-Center, Ruhr-University, Bochum, Germany.
³ Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Dortmund, Germany. corinna.henkel@isas.de.

PMID: 26700046
DOI: 10.1007/978-1-4939-3341-9_10

Abstract

Matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is a technique to visualize molecular features of tissues based on mass detection. This chapter focuses on MALDI MSI of peptides and provides detailed operational instructions for sample preparation of cryoconserved and formalin-fixed paraffin-embedded (FFPE) tissue. Besides sample preparation we provide protocols for the MALDI measurement, tissue staining, and data analysis. On-tissue digestion and matrix application are described for two different commercially available and commonly used spraying devices: the SunCollect (SunChrom) and the ImagePrep (Bruker Daltonik GmbH).

Keywords: Cryoconserved; Formalin-fixed paraffin-embedded (FFPE); ImagePrep; MALDI imaging; MSI; Matrix application; Peptide; SunCollect; Trypsin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cytological Techniques*
Humans
Peptides / chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Peptides