Five kinds of commercially available ELISA kits (acetamiprid, azoxystrobin, chlorothalonil, fenitrothion and imidacloprid) were validated for determination of pesticide residues in vegetables and fruits. The reaction characteristics were also examined to evaluate their influence on the determinations. The trueness value was 91-162%, the repeatability was 2.1-16.2%, and the reproducibility was 4.0-20.3%. The desired values were achieved for 18 among 30 combinations (60%) of the ELISA kits and the agricultural products examined. A standard curve was necessary for each of the ELISA examinations. The matrix of the agricultural products and pipetting skill of the lab technician both influenced the measurment results.