Target-selective photo-degradation of AFP-L3 and selective photo-cytotoxicity against HuH-7 hepatocarcinoma cells using an anthraquinone-PhoSL hybrid

Mai Okuyama; Haruna Ueno; Yuka Kobayashi; Hirokazu Kawagishi; Daisuke Takahashi; Kazunobu Toshima

doi:10.1039/c5cc09542e

Target-selective photo-degradation of AFP-L3 and selective photo-cytotoxicity against HuH-7 hepatocarcinoma cells using an anthraquinone-PhoSL hybrid

Chem Commun (Camb). 2016 Feb 4;52(10):2169-72. doi: 10.1039/c5cc09542e.

Authors

Mai Okuyama¹, Haruna Ueno¹, Yuka Kobayashi², Hirokazu Kawagishi³, Daisuke Takahashi¹, Kazunobu Toshima¹

Affiliations

¹ Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan. toshima@applc.keio.ac.jp.
² J-Oil Mills, Inc., Yokohama 245-0064, Japan.
³ Research Institute of Green Science and Technology, Shizuoka University, Shizuoka 422-8529, Japan.

PMID: 26698630
DOI: 10.1039/c5cc09542e

Abstract

A purposefully-designed anthraquinone-Pholiota squarrosa lectin (PhoSL) hybrid effectively degraded α-fetoprotein-L3 (AFP-L3) associated with liver cancer. Degradation was achieved under light irradiation in the absence of any additives and under neutral pH conditions. Moreover, the hybrid effectively exhibited selective photo-cytotoxicity against HuH-7 hepatocarcinoma cells upon photo-irradiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anthraquinones / chemistry*
Carcinoma, Hepatocellular / chemistry*
Carcinoma, Hepatocellular / metabolism
Cell Line, Tumor
Fetal Proteins / chemistry*
Fetal Proteins / metabolism
Humans
Lectins / chemistry*
Lectins / metabolism
Liver Neoplasms / chemistry*
Liver Neoplasms / metabolism*
Molecular Sequence Data
Pholiota / chemistry*
Pholiota / metabolism
Photolysis
Protein Binding

Substances

Anthraquinones
Fetal Proteins
Lectins