Investigation of bacterial diversity of hot springs of Odisha, India

Rajesh Kumar Sahoo; Enketeswara Subudhi; Mohit Kumar

doi:10.1016/j.gdata.2015.09.018

Investigation of bacterial diversity of hot springs of Odisha, India

Genom Data. 2015 Sep 18:6:188-90. doi: 10.1016/j.gdata.2015.09.018. eCollection 2015 Dec.

Authors

Rajesh Kumar Sahoo¹, Enketeswara Subudhi¹, Mohit Kumar²

Affiliations

¹ Centre Of Biotechnology, Siksha O Anusandhan University, Bhubaneswar, Odisha, India.
² Biotechnology and Bioinformatics, NIIT University, Neemrana, Rajasthan, India.

Abstract

16S rRNA deep sequencing analysis, targeting V3 region was performed using Illumina bar coded sequencing. Sediment samples from two hot springs (Atri and Taptapani) were collected. Atri and Taptapani metagenomes were classified into 50 and 51 bacterial phyla. Proteobacteria (45.17%) dominated the Taptapani sample metagenome followed by Bacteriodetes (23.43%) and Cyanobacteria (10.48%) while in the Atri sample, Chloroflexi (52.39%), Nitrospirae (10.93%) and Proteobacteria (9.98%) dominated. A large number of sequences remained taxonomically unresolved in both hot springs, indicating the presence of potentially novel microbes in these two unique habitats thus unraveling the importance of the current study. Metagenome sequence information is now available at NCBI, SRA database accession no. SRP057428.

Keywords: 16S rRNA; Atri; Bacterial community; Hot springs; Illumina sequencing; Taptapani.