Ultrasensitive Detection of Testosterone Using Microring Resonator with Molecularly Imprinted Polymers

Yangqing Chen; Yong Liu; Xiaodan Shen; Zhimin Chang; Longhua Tang; Wen-Fei Dong; Mingyu Li; Jian-Jun He

doi:10.3390/s151229877

Ultrasensitive Detection of Testosterone Using Microring Resonator with Molecularly Imprinted Polymers

Sensors (Basel). 2015 Dec 15;15(12):31558-65. doi: 10.3390/s151229877.

Authors

Yangqing Chen¹, Yong Liu², Xiaodan Shen³, Zhimin Chang⁴, Longhua Tang⁵, Wen-Fei Dong⁶, Mingyu Li⁷, Jian-Jun He⁸

Affiliations

¹ State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, Zhejiang, China. chenyangqing@ioe-zju.org.
² State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, Zhejiang, China. liuyong@ioe-zju.org.
³ State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, Zhejiang, China. 3110102785@zju.edu.cn.
⁴ CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, Jiangsu, China. changzm@sibet.ac.cn.
⁵ State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, Zhejiang, China. lhtang@zju.edu.cn.
⁶ CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, Jiangsu, China. wenfeidong@sibet.ac.cn.
⁷ State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, Zhejiang, China. limy@zju.edu.cn.
⁸ State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, Zhejiang, China. jjhe@zju.edu.cn.

Abstract

We report ultrasensitive and highly selective detection of testosterone based on microring resonance sensor using molecularly imprinted polymers (MIP). A silicon-on-insulator (SOI) micoring resonator was modified by MIP films (MIPs) on a surface. The MIPs was synthesized by thermopolymerization using methacrylic acid as functional monomer and ethylene glycol dimethacrylate as crosslinking agent. The concentration of detected testosterone varies from 0.05 ng/mL to 10 ng/mL. The detection limit reaches 48.7 pg/mL. Ultrahigh sensitivity, good specificity and reproducibility have been demonstrated, indicating the great potential of making a cost effective and easy to operate lab-on-Chip and down scaling micro-fluidics devices in biosensing.

Keywords: Microring resonator; Molecularly imprinted polymers; Testosterone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / instrumentation*
Biosensing Techniques / methods
Equipment Design
Limit of Detection
Molecular Imprinting / methods*
Reproducibility of Results
Testosterone / analysis*

Substances

Testosterone