Biotransformation and biomethylation of arsenic by Shewanella oneidensis MR-1

Abstract

The resistance of Shewanella oneidensis MR-1 to toxic arsenic was investigated by measuring the growth of the bacteria in the presence of As(III) and As(V) in different growth media. The bacteria were shown to biotransform arsenic through the partial methylation of inorganic arsenic into methylated metabolites. This biotransformation of inorganic arsenic by S. oneidensis MR-1 was affected by the methyl donor, the composition of the medium, and the presence of Fe(III). The relative content of methylated arsenic in the medium containing S-adenosyl methionine as the methyl donor was greater than that in the medium containing methylcobalamin. The biotransformation process driven by Fe-reducing bacteria, and occurred in combination with microbially mediated As-Fe reduction in the presence of Fe(III). The results demonstrate that S. oneidensis MR-1 methylates inorganic arsenic into less toxic organoarsenic compounds. This process has potential applications in the bioremediation of environmental arsenic, and the results provide new insights into the control of in situ arsenic pollution.

Keywords: Arsenic; Biomethylation; Biotransformation; Shewanella oneidensis MR-1.