Physical and chemical properties of an antimicrobial Bis-GMA free dental resin with quaternary ammonium dimethacrylate monomer

Qi-Ting Huang; Jing-Wei He; Zheng-Mei Lin; Fang Liu; Lippo V J Lassila; Pekka K Vallittu

doi:10.1016/j.jmbbm.2015.10.028

Physical and chemical properties of an antimicrobial Bis-GMA free dental resin with quaternary ammonium dimethacrylate monomer

J Mech Behav Biomed Mater. 2016 Mar:56:68-76. doi: 10.1016/j.jmbbm.2015.10.028. Epub 2015 Nov 11.

Authors

Qi-Ting Huang¹, Jing-Wei He², Zheng-Mei Lin³, Fang Liu⁴, Lippo V J Lassila⁵, Pekka K Vallittu⁶

Affiliations

¹ Department of Conservative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology, Sun Yat-Sen University, Guangzhou 510055, China; Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-Sen University, Guangzhou 510055, China; Department of Biomaterials Science and Turku Clinical Biomaterials Centre - TCBC, Institute of Dentistry, University of Turku, 20520 Turku, Finland.
² Department of Biomaterials Science and Turku Clinical Biomaterials Centre - TCBC, Institute of Dentistry, University of Turku, 20520 Turku, Finland; College of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.
³ Department of Conservative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology, Sun Yat-Sen University, Guangzhou 510055, China; Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-Sen University, Guangzhou 510055, China. Electronic address: linzhm@mail.sysu.edu.cn.
⁴ College of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.
⁵ Department of Biomaterials Science and Turku Clinical Biomaterials Centre - TCBC, Institute of Dentistry, University of Turku, 20520 Turku, Finland; Faculty of Health and Well-being, Turku University of Applied Sciences, 20520 Turku, Finland.
⁶ Department of Biomaterials Science and Turku Clinical Biomaterials Centre - TCBC, Institute of Dentistry, University of Turku, 20520 Turku, Finland; City of Turku Welfare Division, Oral Health Care, 20520 Turku, Finland.

PMID: 26688422
DOI: 10.1016/j.jmbbm.2015.10.028

Abstract

The objective of this study was to evaluate the antibacterial activity, physicochemical properties of the quaternary ammonium dimethacrylate monomer N,N-bis[2-(3-(methacryloyloxy)propanamido)-ethyl]-N-methylhexadecyl ammonium bromide (IMQ-16) containing diurethane dimethacrylate (UDMA)/tricyclodecane dimethanol diacrylate (SR833s) resin system and compare with bisphenylglycidyl dimethacrylate (Bis-GMA)/triethylene glycol dimethacrylate (TEGDMA) resin system. It was hypothesized that the physical and chemical properties of the experimental polymers would be comparable with Bis-GMA/TEGDMA polymer and IMQ-16 monomer could endow the UDMA/SR833s resin with antibacterial activity. Double bond conversion (DC) was measured using Fourier transform infrared spectroscopy (FTIR). Mechanical properties including flexural strength (FS) and flexural modulus (FM) were measured by three-point bending test with bars of 2mm×2mm×25mm. Water sorption (WS) and solubility (WSL) were also investigated. Antibacterial activity of obtained polymers against Streptococcus mutans Ingbitt (S. mutans) was tested through direct contact test (DCT). The presence of antibacterial activity due to soluble components was also investigated by agar diffusion test (ADT). All of the polymers containing IMQ-16 exhibited improvements in WS and WSL, while maintaining equivalent DC and FS relative to the Bis-GMA/TEGDMA control system. Incorporation of 17% and 20% of IMQ-16 into UDMA/SR833s resin reduced the viable counts of S. mutans after incubation on the surface of the materials and produced no inhibition zones around the cured discs in ADT. UDMA/SR833s resin system is promising to formulate an antibacterial polymer with equivalent or even higher physicochemical properties relative to Bis-GMA/TEGDMA formulation. IMQ-16 is capable to endow UDMA/SR833s resin system with significant antibacterial activity when the mass ratio is 17% or 20%.

Keywords: Antibacterial activity; Dental resin; Dimathacrylate; Physicochemical properties; Polymer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / chemistry*
Anti-Bacterial Agents / pharmacology*
Bisphenol A-Glycidyl Methacrylate / chemistry
Chemical Phenomena*
Methacrylates / chemistry*
Polymerization
Quaternary Ammonium Compounds / chemistry*
Resins, Synthetic / chemistry*
Resins, Synthetic / pharmacology*
Streptococcus mutans / drug effects

Substances

Anti-Bacterial Agents
Methacrylates
Quaternary Ammonium Compounds
Resins, Synthetic
methacrylic acid
Bisphenol A-Glycidyl Methacrylate