Protumorigenic M2-like phenotype cell infiltration in the melanotic neuroectodermal tumor of infancy

Luciana Strieder; Román Carlos; Jorge Esquiche León; Alfredo Ribeiro-Silva; Victor Costa; Estela Kaminagakura

doi:10.1016/j.oooo.2015.09.015

Protumorigenic M2-like phenotype cell infiltration in the melanotic neuroectodermal tumor of infancy

Oral Surg Oral Med Oral Pathol Oral Radiol. 2016 Feb;121(2):173-9. doi: 10.1016/j.oooo.2015.09.015. Epub 2015 Dec 10.

Authors

Luciana Strieder¹, Román Carlos², Jorge Esquiche León³, Alfredo Ribeiro-Silva⁴, Victor Costa¹, Estela Kaminagakura⁵

Affiliations

¹ Master Student of Oral Biopathology, Institute of Science and Technology, UNESP-Univ Estadual Paulista, School of Dentistry, São José dos Campos, Brazil.
² Director of Centro Clínico de Cabeza y Cuello, Guatemala City, Guatemala.
³ Professor of Oral Pathology, Department of Stomatology, Public Oral Health and Forensic Dentistry, University of São Paulo, Ribeirão Preto School of Dentistry, Ribeirão Preto, Brazil.
⁴ Department of Pathology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
⁵ Professor of Stomatology, Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, UNESP-Univ Estadual Paulista, School of Dentistry, São José dos Campos, Brazil. Electronic address: estela@fosjc.unesp.br.

PMID: 26686956
DOI: 10.1016/j.oooo.2015.09.015

Abstract

Objective: The aim of this study is to report 2 cases of melanotic neuroectodermal tumor of infancy (MNTI), emphasizing the analysis of intratumoral immune cells by immunohistochemistry.

Study design: Case 1: A 6-month-old girl presented with a 3-cm tumor in the anterior region of the left maxilla. Case 2: A 4-month-old boy presented with a 4-cm tumor in the anterior region of the left maxilla. Microscopically, case 1 had predominantly neuroblast-like cells supported by fibrillary neuropil-like stroma arranged in an alveolar pattern, whereas case 2 exhibited scattered melanocyte-like and neuroblast-like cells supported by fibrovascular stroma. A large immunohistochemical panel for characterizing intratumoral macrophage and dendritic cell subsets was performed.

Results: Immunohistochemical analysis indicated positivity for HLA-DR, XIIIa, CD68, and CD163 (range 6%-50%) mainly on the fibrovascular stroma, suggesting M2 macrophage-like cell phenotype. CD138 was overexpressed in the tumor stroma.

Conclusions: Results suggest the involvement of M2-polarized macrophages in the MNTI pathogenesis, which may act by modulating tumor growth and/or tumor stromal remodeling.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Female
Humans
Immunohistochemistry
Infant
Macrophages / pathology*
Male
Maxillary Neoplasms / pathology*
Neuroectodermal Tumor, Melanotic / pathology*
Phenotype