Rapid Reversible Photoswitching of Integrin-Mediated Adhesion at the Single-Cell Level

Adv Mater. 2016 Mar 2;28(9):1799-802. doi: 10.1002/adma.201504394. Epub 2015 Dec 21.

Abstract

Rapid and reversible photoswitching of cell adhesion is achieved by c(RGDfK)-azobenzenes embedded in a poly(ethylene glycol) background on surfaces. The light-induced cis-trans-isomerization of the azobenzene enables switching of cell adhesion on the surface. Reversibility of switching over several consecutive switching cycles is demonstrated by single-cell force spectroscopy.

Keywords: atomic force microscopy; cell adhesion; integrin; photoswitching; surface chemistry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azo Compounds / chemistry
  • Azo Compounds / metabolism
  • Azo Compounds / pharmacology
  • Cell Adhesion / drug effects
  • Cell Line
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Integrins / metabolism*
  • Light*
  • Peptides, Cyclic / chemistry
  • Single-Cell Analysis
  • Surface Properties

Substances

  • Azo Compounds
  • Integrins
  • Peptides, Cyclic
  • cyclic (arginyl-glycyl-aspartyl-phenylalanyl-lysyl)
  • azobenzene