Infection studies of nontarget mammalian cell lines with Bombyx mori macula-like virus

Katsuhisa Innami; Takahiro Aizawa; Toshihiro Tsukui; Susumu Katsuma; Shigeo Imanishi; Hideki Kawasaki; Masashi Iwanaga

doi:10.1016/j.jviromet.2015.12.002

Infection studies of nontarget mammalian cell lines with Bombyx mori macula-like virus

J Virol Methods. 2016 Mar:229:24-6. doi: 10.1016/j.jviromet.2015.12.002. Epub 2015 Dec 10.

Authors

Katsuhisa Innami¹, Takahiro Aizawa¹, Toshihiro Tsukui², Susumu Katsuma³, Shigeo Imanishi⁴, Hideki Kawasaki¹, Masashi Iwanaga⁵

Affiliations

¹ Department of Agrobiology and Bioresources, Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya-shi, Tochigi 321-8505, Japan.
² Animal Life Science Laboratory, Nippon Zenyaku Kogyo Co., Ltd., 1-1 Tairanoue Sasagawa, Asaka, Koriyama, Fukushima 963-0196, Japan.
³ Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
⁴ Genebank, National Institute of Agrobiological Science, Kannondai 2-1-2, Tsukuba-shi, Ibaraki 305-8602, Japan.
⁵ Department of Agrobiology and Bioresources, Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya-shi, Tochigi 321-8505, Japan. Electronic address: iwanaga@cc.utsunomiya-u.ac.jp.

PMID: 26685801
DOI: 10.1016/j.jviromet.2015.12.002

Abstract

Bombyx mori-derived cell lines are generally used for Bombyx mori nucleopolyhedrovirus (BmNPV)-based baculovirus expression vector system (BEVS). However, almost all of the B. mori-derived cell lines are persistently infected with Bombyx mori macula-like virus (BmMLV). In this study, nontarget mammalian cell lines were exposed to BmMLV, and their susceptibility was investigated. Real-time PCR showed that viral RNA in virus-inoculated nine mammalian cell lines decreased sharply at 7 days postinfection. Also, there was no significant effect on cell viability of mammalian cells after inoculation with BmMLV. These findings indicate that mammalian cell lines used in this study are not permissive to BmMLV, and BmMLV contamination might not affect the safety aspect of BmNPV-based BEVS.

Keywords: BEVS; BmMLV; BmNPV; Maculavirus; Mammalian cell line.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bombyx / virology*
Cell Line
Cell Survival
Host Specificity*
Mammals
RNA, Viral / analysis
Real-Time Polymerase Chain Reaction
Tymoviridae / growth & development*
Virus Cultivation*

Substances

RNA, Viral