Evaluation of the BinaxNOW® Streptococcus pneumoniae antigen test on fresh, frozen and concentrated urine samples in elderly patients with and without community-acquired pneumonia

Annika Saukkoriipi; Thierry Pascal; Arto A Palmu

doi:10.1016/j.mimet.2015.12.007

Evaluation of the BinaxNOW® Streptococcus pneumoniae antigen test on fresh, frozen and concentrated urine samples in elderly patients with and without community-acquired pneumonia

J Microbiol Methods. 2016 Feb:121:24-6. doi: 10.1016/j.mimet.2015.12.007. Epub 2015 Dec 9.

Authors

Annika Saukkoriipi¹, Thierry Pascal², Arto A Palmu³

Affiliations

¹ Department of Health Protection, National Institute for Health and Welfare, Oulu, Finland. Electronic address: annika.saukkoriipi@thl.fi.
² GSK Vaccines, Wavre, Belgium.
³ Department of Health Protection, National Institute for Health and Welfare, Tampere, Finland.

PMID: 26684855
DOI: 10.1016/j.mimet.2015.12.007

Abstract

We evaluated the BinaxNOW® urine antigen test in elderly. For fresh un-concentrated urine samples, the sensitivity for pneumococcal pneumonia was 63% and specificity 97%. After freezing and concentration, the results comparable to positive control line in intensity at 60 min gave high sensitivity (81%) with no loss in specificity (96%).

Keywords: Community-acquired pneumonia; Streptococcus pneumoniae; Urine antigen.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Antigens, Bacterial / urine*
Bacteriological Techniques / instrumentation
Bacteriological Techniques / methods*
Community-Acquired Infections / diagnosis
Community-Acquired Infections / microbiology
Community-Acquired Infections / urine*
Diagnostic Tests, Routine / methods
Freezing
Humans
Pneumonia, Pneumococcal / diagnosis
Pneumonia, Pneumococcal / microbiology
Pneumonia, Pneumococcal / urine*
Streptococcus pneumoniae / immunology
Streptococcus pneumoniae / isolation & purification*
Urine / chemistry
Urine / microbiology

Substances

Antigens, Bacterial